Cryodehydration is an efficient and cost-effective method for preserving the gross morphology of anatomical specimens. However, repeated freezing and thawing causes substantial cellular damage due to microruptures of the cell membrane. Objectives: Therefore, the present study was designed to investigate the role of cryoprotectant and controlled thawing protocols in optimizing cryodehydration technique for preparing cryodehydrated specimens, with particular focus on the preservation of histoarchitectural integrity. Method: To conduct this study, four healthy goats were used, labelled as G1-control, G2-formalin preserved, G3-cryodehydrated using routine protocol, and G4-cryodehydrated using glycerol as cryoprotectant and controlled thawing (4 °C). Results: Although the G4 specimens required more freeze-thaw cycle, both protocols (G3 and G4) resulted in excellent preservation of color, texture, and morphology. The specimens were soft, dry, odorless, lightweight, and durable. In the histological study, G2 yielded better histoarchitectural preservation resembling to the control while massive histoarchitectural alterations were observed in G3. These included noticeable thinning of the heart wall, increased spaces between cardiac myofibers, decreased bronchial and bronchiolar circumference and reduced alveolar surface area in the lungs, loss of hepatocytes and widened sinusoidal spaces in the liver, damage to both renal tubules and glomeruli, loss of lymphoid tissue in the spleen, and shrinkage of skeletal myofibers. In contrast, G4 demonstrated better preservation of histoarchitecture, maintaining cellular integrity along with distinct cytoplasmic and nuclear clarity. Conclusion: Based on these findings, it can be concluded that the use of glycerol as a cryoprotectant, in combination with controlled thawing at 4°C, enhances the efficacy of the cryodehydration technique in preserving tissue histoarchitecture.