The Journal of Plastination

Published in J. Int. Soc. Plast. 9(1): 37 (1995)

The Use of E-20 Red Resin For Casting Anatomical Cavities

AUTHORS:
WF Pretorius , and Dr HJ Geyer
affiliations:

Department of Anatomy and Cell Morphology, University of the Orange Free State, Republic of South Africa.

ABSTRACT:

The first recorded example of the casting of anatomical cavities was that of Leonardo Da Vinci (1452 - 1519). Molten wax was used to cast the ventricles of the brain. By doing so Da Vinci developed a new dimension in the study of anatomical cavities by filling them with a solidifying agent.

Looking at Biodur E20 resin in comparison to wax, we find that they have common characteristics. They both solidify, but more important for the technician, is the fact that heat plays a role in the consistency of these products.

When cold, E20 has a high viscosity. At room temperature it has the consistency of honey and at 50°C it pours like water. Using this phenomenon one can inject micro anatomical cavities.

KEY WORDS:

E20; casts; injection; cavities; Ventricles

*CORRESPONDENCE TO:

WF Pretorius Dept. Anatomy and Cell Morphology, University of the Orange Free State, Republic of South Africa.

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Article Statistics

Volume: 9
Issue: 1
Allocation-id: 0000

Submitted Date:September 13, 1995
Accepted Date: November 24, 1995
Published Date: December 31, 1995

DOI Information:       https://doi.org/10.56507/JQTR9706

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The Journal of Plastination (June 20, 2024) The Use of E-20 Red Resin For Casting Anatomical Cavities. Retrieved from https://journal.plastination.org/articles/the-use-of-e-20-red-resin-for-casting-anatomical-cavities/.
"The Use of E-20 Red Resin For Casting Anatomical Cavities." The Journal of Plastination - June 20, 2024, https://journal.plastination.org/articles/the-use-of-e-20-red-resin-for-casting-anatomical-cavities/
The Journal of Plastination - The Use of E-20 Red Resin For Casting Anatomical Cavities. [Internet]. [Accessed June 20, 2024]. Available from: https://journal.plastination.org/articles/the-use-of-e-20-red-resin-for-casting-anatomical-cavities/
"The Use of E-20 Red Resin For Casting Anatomical Cavities." The Journal of Plastination [Online]. Available: https://journal.plastination.org/articles/the-use-of-e-20-red-resin-for-casting-anatomical-cavities/. [Accessed: June 20, 2024]

INTRODUCTION

The first recorded example of the casting of anatomical cavities was that of Leonardo Da Vinci (1452 - 1519). Molten wax was used to cast the ventricles of the brain. By doing so Da Vinci developed a new dimension in the study of anatomical cavities by filling them with a solidifying agent.

Looking at Biodur E20 resin in comparison to wax, we find that they have common characteristics. They both solidify, but more important for the technician, is the fact that heat plays a role in the consistency of these products.

When cold, E20 has a high viscosity. At room temperature it has the consistency of honey and at 50°C it pours like water. Using this phenomenon one can inject micro anatomical cavities.

AIM:

The aim of this method is to produce a corrosion cast of the glomeruli of the kidneys of a rat.

MATERIALS AND METHODS

The abdominal aorta was blocked distally to the kidneys by tying surgical silk tightly around it. A canula was inserted into the thoracic aorta and the blood was washed out of the kidneys using saline solution with heparin.

The rat was submerged in a water bath at 50°C for + 20 minutes. A beaker containing the E20 was standing in a water bath at the same temperature.

Injection of E20 was completed using a 10 cc syringe (preheated) until the kidneys were visibly full. One important point to remember is that by heating the resin the setting time is reduced dramatically. No time should be wasted after mixing the catalyst with the resin. After curing of the resin the soft tissue was removed (macerated) with KOH ( 4%) and studied with a scanning electron microscope.

The glomeruli were completely filled with E20, and the arteries and veins were clearly visible.

In conclusion we found E20 resin an exceptionally suitable resin to study the ultrastructure of anatomical cavities.

A scanning elecyron micrograph of a glomerulus of a rat kidney filled with E20 resin. (X470)

REFERENCES

Microvascular Corrosion Casting in Scanning Electrone Microscopy (first edition), Springer, Berlin-Heidelberg-Wien (1992) pp. 3–9 (a), p. 58 (b)
Online ISSN: 2311-777X
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